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	Provedor de dados Biol. Res. (2) Autor BULL,PAULINA (2) CHÁVEZ,RENATO (2) EYZAGUIRRE,JAIME (2) PEIRANO,ALESSANDRA (2) SCHACHTER,KAREN (2) ALMARZA,CAROLINA (1) NAVARRO,CLAUDIO (1) Mais... Palavra-chave Penicillium purpurogenum (2) Acetyl xylan esterase (1) Family 10 endoxylanases (1) Gene expression (1) Gene structure (1) Tipo do documento Journal article (2) Ano 2004 (1) 2001 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Structure analysis of the endoxylanase A gene from Penicillium purpurogenum Biol. Res. CHÁVEZ,RENATO; ALMARZA,CAROLINA; SCHACHTER,KAREN; PEIRANO,ALESSANDRA; BULL,PAULINA; EYZAGUIRRE,JAIME. Penicillium purpurogenum produces several endoxylanases, two of which (XynA and XynB) have been purified and characterized. XynB has been sequenced, and it belongs to glycosyl hydrolase family 11. In this publication we report the structure of the xynA gene. The amino terminal sequence of the protein was determined and this allowed the design of oligonucleotides for use in polymerase chain reactions. Different polymerase chain reaction strategies were used to amplify and sequence the entire cDNA and the gene. The gene has an open reading frame of 1450 base pairs, including 8 introns with an average length of 56 base pairs each. Only one copy of this gene is present in the P. purpurogenum genome as shown by Southern blot. The gene encodes a protein of 329... Tipo: Journal article Palavras-chave: Family 10 endoxylanases; Gene structure; Penicillium purpurogenum. Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000300009 The acetyl xylan esterase II gene from Penicillium purpurogenum is differentially expressed in several carbon sources, and tightly regulated by pH Biol. Res. CHÁVEZ,RENATO; SCHACHTER,KAREN; NAVARRO,CLAUDIO; PEIRANO,ALESSANDRA; BULL,PAULINA; EYZAGUIRRE,JAIME. The expression of the acetyl xylan esterase II (axeII) gene from Penicillium purpurogenum is repressed by glucose and induced by xylan, as well as to a small degree by xylose and xylitol. This gene is expressed at neutral pH, but not under alkaline or acidic conditions, in agreement with previous findings for other xylanolytic genes of this organism. This is the first report showing pH regulation of an axe gene. Tipo: Journal article Palavras-chave: Acetyl xylan esterase; Gene expression; Penicillium purpurogenum. Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100011 Registros recuperados: 2 Primeira ... 1 ... Última

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